What is Triton X-100 Non-Ionic Surfactant

Triton X-100 (CAS 9002-93-1) is a widely used non-ionic surfactant known for its effective emulsifying and solubilizing properties. Chemically, it is polyethylene glycol p-(1,1,3,3-tetramethylbutyl)-phenyl ether, a compound featuring both hydrophilic and hydrophobic components that enable it to interact with a broad range of substances.

Chemical Composition and Structure

Core structure: A polyethylene glycol (PEG) chain forms the hydrophilic segment.
Hydrophobic group: An aromatic phenyl ring substituted with a bulky 1,1,3,3-tetramethylbutyl group contributes to its non-polar character.
Molecular formula: (C14H22O)(C2H4O)n, where n indicates the average number of ethylene oxide units.

This amphiphilic structure allows Triton X-100 to reduce surface tension, making it highly effective in breaking down lipid membranes without denaturing proteins.

Physical Properties

Appearance: Clear, viscous liquid
Specific gravity: Approximately 1.07 at 25°C
Viscosity: Moderate viscosity suitable for easy handling and mixing
Solubility: Highly soluble in water, ethanol, and other organic solvents

Surfactant Characteristics

Non-ionic nature means Triton X-100 carries no net charge, resulting in mild interaction with biological molecules.
The hydrophilic polyethylene oxide chain attracts water molecules, promoting solubility.
The hydrophobic aromatic group interacts with lipids and non-polar phases, enabling membrane disruption and emulsification.

Comparison with Related Compounds

Compared to ionic surfactants, Triton X-100’s non-ionic profile causes less protein denaturation, making it preferred for biochemical research and sensitive molecular biology applications. It shares similarities with other non-ionic surfactants such as NP-40 but is distinguished by its specific aromatic group and oxyethylene chain length, offering unique balance in solubilization and mildness.

For understanding alternatives to Triton X-100 and compliance with current regulations, you may also explore our range of eco-friendly detergents and surfactants.

Key Applications of Triton X-100

Triton X-100 (CAS 9002-93-1) is a widely used non-ionic surfactant with versatile applications across biochemical research, molecular biology, and industrial sectors. Its unique ability to solubilize membranes and interact with both hydrophilic and hydrophobic substances makes it essential in many workflows.

Biochemical Research

Membrane Protein Solubilization

Triton X-100 effectively disrupts lipid bilayers, making it a top choice for solubilizing membrane proteins without denaturing them. This is crucial for studying protein structure and function.
Cell Lysis and Organelle Extraction

It gently lyses cells, allowing researchers to extract intact organelles and preserve the activity of sensitive biomolecules. This makes it reliable for downstream biochemical assays.

Molecular Biology

DNA Extraction

Used in protocols to permeabilize cell membranes, Triton X-100 aids in releasing DNA while maintaining nucleic acid integrity.
Immunofluorescent Microscopy

It helps permeabilize cells for antibody access, improving staining quality without damaging cellular structures.
Transfection

Triton X-100 facilitates the introduction of genetic material into cells by weakening cell membranes, supporting efficient gene delivery methods.

Industrial Uses

Pharmaceutical Emulsifier

Acts as a stable emulsifying agent in drug formulations, improving the solubility and bioavailability of active ingredients.
Dispersion Agent in Soft Composites

Enhances the uniform spread of components in composite materials, leading to better product performance.
Component in Cleaning Solutions

Its surfactant properties allow it to break down oils and greases, making it a valuable ingredient in high-performance cleaners.

Specialized Applications

Active Ingredient in Influenza Vaccines

Employed to inactivate viruses and boost vaccine efficacy without compromising safety.
Vinyl Record Cleaning Fluids

Its mild detergent action removes dirt and grime gently, preserving vinyl quality during cleaning.

By covering these key applications, Triton X-100 remains a versatile and trusted compound across multiple critical fields, demonstrating consistent performance for biochemical research chemicals, molecular biology reagents, and industrial formulations in the U.S. market.

Triton X-100 Benefits and Features

Triton X-100 (CAS 9002-93-1) offers multiple advantages that make it a go-to choice for both biochemical research and industrial applications in the United States.

Mild and Non-Denaturing Detergent

Gentle on biological samples: Triton X-100 is a mild, non-ionic surfactant that does not denature proteins or damage delicate cell structures. This makes it ideal for sensitive biological processes like cell lysis, membrane protein solubilization, and organelle extraction.
It preserves the native structure of biomolecules, which is critical when working on molecular biology and biochemical research.

High Solubility and Versatility

It dissolves easily in water, ethanol, and many organic solvents, providing flexibility across various lab and industrial settings.
This high solubility supports its use in diverse formulations, from DNA extraction buffers to cleaning solutions in pharmaceutical production.

Stability and Reliability

When stored properly in a cool, dry place, Triton X-100 remains stable for years, ensuring consistent performance batch after batch.
This stability is crucial for long-term projects and large-scale operations requiring dependable supply and quality.

Enhances Protein Extraction and Virus Inactivation

Widely used in biopharmaceutical applications, Triton X-100 improves the efficiency of protein extraction, aiding researchers in obtaining high-quality membrane proteins.
It also plays a key role in virus inactivation, making it valuable in vaccine manufacturing and viral research.

of Key Benefits

Non-denaturing surfactant preserves biological activity
High solubility in water and solvents for versatile use
Long-term stability for reliable results
Enhances protein extraction and virus inactivation for biopharma

Choosing Triton X-100 ensures you get a reliable, effective surfactant well-suited to the needs of US-based research labs and industries focused on biochemical and pharmaceutical applications.

Regulatory and Environmental Considerations for Triton X-100

Triton X-100 (CAS 9002-93-1) faces increasing scrutiny under EU REACH regulations due to its ecotoxic degradation products. These breakdown products can persist in the environment, posing risks to aquatic life and ecosystems. Because of these concerns, stricter restrictions are being placed on the use, disposal, and manufacturing of Triton X-100 within the European Union.

Need for Eco-Friendly Alternatives

This regulatory pressure has accelerated the search for eco-friendly detergents that deliver similar performance without harmful environmental effects. Industries relying on Triton X-100, especially in biochemical research and industrial cleaning, are actively exploring sustainable substitutes that comply with evolving safety standards without compromising efficiency.

Blissam’s Commitment to Compliance and Sustainability

At Blissam, we understand the importance of maintaining regulatory compliance while supporting environmental stewardship. We:

Rigorously ensure all Triton X-100 supplied meets current regulatory standards for both the U.S. and international markets.
Support customers by offering detailed documentation and lot-specific Certificates of Analysis to facilitate compliance.
Invest in sustainable sourcing practices to minimize environmental impact.
Provide guidance and alternatives for clients seeking Triton X-100 replacements compatible with global regulations.

Choosing Blissam means partnering with a trusted supplier who puts both compliance and sustainability at the forefront—helping your business stay ahead in a changing regulatory landscape.

Why Choose Blissam for Triton X-100 CAS 9002-93-1

When it comes to sourcing Triton X-100 CAS 9002-93-1 for your research or industrial needs, Blissam stands out as a trusted partner. Here’s why:

High Purity and Reliable Quality

Blissam provides high-purity Triton X-100, ideal for sensitive biochemical research, molecular biology, and industrial uses. Every batch undergoes rigorous quality control to meet strict standards, backed by lot-specific Certificates of Analysis to guarantee consistency and purity.

Comprehensive Support for Regulatory Compliance

With increasing restrictions under EU REACH regulations and growing demand for eco-friendly products, Blissam offers expert guidance on compliance issues. We help you navigate regulatory requirements and also provide information on Triton X-100 alternatives when needed.

Fast and Global Supply Chain

Our well-established global supply chain ensures fast, reliable delivery across the United States. Whether you need Triton X-100 for labs, pharmaceutical emulsions, or cleaning solutions, Blissam can deliver on time to keep your processes running smoothly.

Expertise and Customized Solutions

Blissam is more than a supplier — we’re a partner. We provide technical support on product handling, safety, and application optimization. Need a custom order or specific packaging? We’re ready to tailor solutions to fit your exact requirements.

Contact Blissam today for a quote or to discuss your Triton X-100 needs. Experience peace of mind with a supplier committed to quality, compliance, and customer support in the U.S. market.

Safety and Handling Guidelines for Triton X-100 CAS 9002-93-1

Toxicity Information

Triton X-100 is moderately toxic if ingested and can cause eye and skin irritation. Direct contact should be avoided to prevent discomfort or injury.

Safety Precautions

To handle Triton X-100 safely:

Wear gloves and eye protection at all times.
Avoid inhaling vapors or allowing the liquid to contact your skin.
Prevent any release into the environment—dispose of spills immediately and responsibly.

Storage Recommendations

Store Triton X-100 in a sealed container.
Keep it in a cool, dry place to maintain product stability and performance over time.
Avoid exposure to excessive heat or direct sunlight, which can degrade the surfactant quality.

Disposal Guidelines

Dispose of Triton X-100 according to local hazardous waste regulations.
Do not pour it down drains or release it into the environment, as it may cause ecological harm due to its degradation products.

Following these safety and handling practices ensures you make the most of Triton X-100’s benefits while protecting yourself and the environment. For U.S. customers, adhering to OSHA guidelines and EPA regulations is essential when using this non-ionic surfactant in research or industrial settings.

Alternatives to Triton X-100

Eco-friendly Substitutes for Triton X-100

Due to increasing EU REACH regulations and environmental concerns, many labs and industries are shifting toward eco-friendly detergents as alternatives to Triton X-100 (CAS 9002-93-1). These substitutes aim to provide similar non-ionic surfactant properties but with less environmental impact, especially concerning biodegradability and toxicity.

Some well-known alternatives include:

Triton X-100 reduced – a modified version designed to reduce harmful degradation byproducts
Octyl glucoside – a biodegradable, mild detergent popular in biochemistry
Tween 20 and Tween 80 – non-ionic surfactants widely used in pharmaceuticals and molecular biology
Brij series surfactants – polyethylene glycol-based detergents with favorable solubility and stability profiles

Comparison of Properties and Applications

When choosing an alternative, it’s important to compare:

Property	Triton X-100	Eco-friendly Alternatives
Non-ionic character	Yes	Yes
Biodegradability	Limited, concerns under EU laws	High, better environmental profile
Protein solubilization	Excellent	Comparable, varies by compound
Stability	Very stable	Generally stable, depends on storage
Regulatory compliance	Restricted in some markets	Fully compliant with REACH

For applications like membrane protein solubilization, cell lysis, and emulsifying pharmaceuticals, these alternatives can often match Triton X-100’s performance with a greener footprint.

Blissam’s Role in Providing Alternative Surfactants

At Blissam, we understand the growing demand for Triton X-100 alternatives that meet strict regulatory compliance in the U.S. and Europe. We offer:

A curated portfolio of high-purity, eco-friendly detergents suitable for biochemical research and industrial use
Expert guidance on selecting the right surfactant based on your application and compliance needs
Support for transitioning from Triton X-100 to safer, sustainable options without compromising on quality or performance

Working with Blissam means you get trusted chemical sourcing with a focus on sustainability, helping your projects meet today’s environmental standards without losing effectiveness. Contact us to explore our range of alternatives and ensure your surfactant choices are both high-quality and regulation-ready.

Frequently Asked Questions about Triton X-100 CAS 9002-93-1

What is the CAS number for Triton X-100

The CAS number for Triton X-100 is 9002-93-1. This unique identifier helps easily locate the specific non-ionic surfactant used in many biochemical and industrial applications.

Is Triton X-100 safe for laboratory use

Triton X-100 is widely used in laboratories, especially for membrane protein solubilization and cell lysis. However, it should be handled following safety guidelines: wear gloves and eye protection and avoid direct contact. It’s moderately toxic if ingested and can irritate skin and eyes, so proper handling and storage are critical for safe use.

What are the alternatives under EU regulations

Due to the EU REACH restrictions on Triton X-100 because of its ecotoxic degradation products, several eco-friendly alternatives are gaining traction. These substitutes offer similar non-ionic surfactant properties while meeting environmental compliance. Blissam provides guidance and supply options for these Triton X-100 alternatives, helping you stay compliant without compromising performance.

How does Blissam ensure product quality

Blissam guarantees high-purity Triton X-100 manufactured under strict quality control. Each batch comes with a Certificate of Analysis to verify specification compliance, ensuring reliable performance for biochemical research chemicals and industrial uses. Our global supply chain ensures consistent product availability for U.S. customers.

How can I order Triton X-100 from Blissam

Ordering Triton X-100 CAS 9002-93-1 from Blissam is straightforward:

Contact our sales team via phone or email for quotes
Request custom orders or bulk pricing based on your project needs
Benefit from fast, reliable shipping across the United States
Get expert advice on regulatory compliance and alternative surfactants

Reach out today to secure your supply of high-quality Triton X-100 for research or industrial applications.

Triton X-100 CAS 9002-93-1